AP2A (AA 1-25) Antikörper

Produktnummer ABIN129553

Hersteller Produkt- Nr.: 600-101-287

Dieser Ziege Polyklonal Antikörper erkennt spezifisch in ELISA, IP und WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für AP2A (AA 1-25) Antikörper (ABIN129553)

Target: AP2A

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 1-25

Hersteller Produkt- Nr.: 600-101-287

Hersteller: Rockland

Verwendungszweck: AP2A Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human AP2A.

Produktmerkmale: Synonyms: goat anti-AP2A Antibody, AP-2 complex subunit alpha-1, Adapter-related protein complex 2 alpha-1 subunit, Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin, Plasma membrane adaptor HA2/AP2, Clathrin assembly protein complex 2 alpha-A large chain, AP2A2, ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899

Aufreinigung: The product was affinity purified from antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of Human AP2A1 and AP2A2 proteins.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 104-107 kDa in size corresponding to AP2A1 or AP2A2 proteins by western blotting in the appropriate cell lysate or extract. Multiple isoforms of each subunit exist for these proteins. The immunogen sequence is found in both AP2A1 and AP2A2. This antibody recognizes a band of approximately 100 kDa in multiple human cell lines and specific reactivity can be blocked with the immunizing peptide.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.7 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: AP2A

Hintergrund: Background: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit.  The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.

Gen-ID: 161, 27477041

UniProt: O95782